DANGER : Software Tool For Genome Editing

  • A team of researchers recently developed a software tool called DANGER (Deleterious
    and Anticipatable Guides Evaluated by RNA-sequencing) analysis that provides a way for
    the safer design of genome editing in all organisms.
  • DANGER analysis is a new software tool that makes genome editing safer by overcoming
    limitations in current CRISPR technology.
  • Unlike traditional methods that rely on a reference genome, DANGER works by
    conducting risk-averse on- and off-target assessments using RNA-sequencing data.
  • It evaluates phenotypic effects based on gene expression changes caused by deleterious
    off-target sites.
  • The software quantifies the phenotypic risk at the gene ontology level without the need
    for a reference genome.
  • The tool successfully evaluated the phenotypic effects of gene editing in human cells and
    zebrafish brains.
  • It can be performed on various organisms, personal human genomes, and atypical
    genomes created by diseases and viruses.
  • The open-source nature of the software allows for its adaptation to different genome
    editing systems beyond CRISPR-Cas9.
    Genome editing
  • It is a method that lets scientists change the DNA of many organisms, including plants,
    bacteria, and animals.
  • Editing DNA can lead to changes in physical traits, like eye colour, and disease risk.