- A team of researchers recently developed a software tool called DANGER (Deleterious
and Anticipatable Guides Evaluated by RNA-sequencing) analysis that provides a way for
the safer design of genome editing in all organisms. - DANGER analysis is a new software tool that makes genome editing safer by overcoming
limitations in current CRISPR technology. - Unlike traditional methods that rely on a reference genome, DANGER works by
conducting risk-averse on- and off-target assessments using RNA-sequencing data. - It evaluates phenotypic effects based on gene expression changes caused by deleterious
off-target sites. - The software quantifies the phenotypic risk at the gene ontology level without the need
for a reference genome. - The tool successfully evaluated the phenotypic effects of gene editing in human cells and
zebrafish brains. - It can be performed on various organisms, personal human genomes, and atypical
genomes created by diseases and viruses. - The open-source nature of the software allows for its adaptation to different genome
editing systems beyond CRISPR-Cas9.
Genome editing - It is a method that lets scientists change the DNA of many organisms, including plants,
bacteria, and animals. - Editing DNA can lead to changes in physical traits, like eye colour, and disease risk.
